The Laboratory was founded in 2010.
The concept of biological integration of the human metabolism and the metabolism of human microbiome forms the basis of our research. The other part of this theory implies the direct participation of the microbial metabolites in the pathogenesis of critical states.
The research is based on the interdisciplinary approach combining efforts of anesthesiologists, intensivists, microbiologists, biochemists, biophysicists, mass spectrometry specialists.
The Laboratory works in a close cooperation with other research institutes and hospitals (The Lomonosov Moscow State University, Institute of Theoretical and Experimental Biophysics of the Russian academy of sciencesRAS, Scientific Research Institute of Epidemiology and Microbiology of the Russian Academy of Medical Sciences, Health Department of the Moscow City, S.P. Botkin City Clinical Hospital, Filatov Children City Clinical Hospital №13 et al.).
Clinical research is focused on the detection of the most important metabolites in the blood of patients which may correlate with the systemic inflammatory response syndrome (SIRS), sepsis, multiple organ failure (MOF) and mortality.
The research of the potential producers of these metabolites among human microbiome and clinically important bacteria (both aerobe and anaerobe) is conducted in the microbiological laboratory.
Metabolic activities of microorganisms are investigated in vitro during conventional cultivation in liquid medium (planctonic form ) and biofilms.
Toxic and signaling effects of these metabolites are evaluated both at cellular and subcellular levels under experimental conditions. The research results make the basis for rethinking of a number of pathogenetic pathwaysof critical conditions (intoxication, catabolism, MOF et al.) taking into account the role of the microbial metabolites.
Gas chromatography and mass spectrometry techniques for metabolic profiling are being developed at the Laboratory. These techniques will be adapted for the clinical laboratory conditions. It will provide practicians with a possibility of detection of the most important microbial metabolites in the blood of patients and thus diagnosing sepsis, monitoring critical conditions and evaluating the treatment effectiveness.
Assessment of the value of the diagnostic tests (microbial DNA, PCT and other biomarkers) is conducted using immunochemical methods and PCR analysis. It allows to provide monitoring in critically ill patients (ventilated newborns, patients with traumatic brain injury and others) using minimal blood sample volume.
The development of algorithms for diagnosing, monitoring and selective metabolism correction is the main practical goal of the Laboratory. Implementation of these algorithms is targeted on improving outcomes in critically ill patients and reducing ICU mortality.
General Resuscitation im.V.A.Negovskogo
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